Dept. of Biotechnology

Biochemistry Laboratory   

This lab provides an in-depth first hand knowledge of various instrumental techniques, with special emphasis on real time spectrometers. Experiments for both qualitative and quantitative analysis of bimolecular proteins like DNA, RNA etc. are carried out with the aid of a wide variety of equipment like colorimeters, spectrophotometers, fluorimeters, etc.,. Separation techniques like paper and thin layer chromatography are also practiced. The special feature of this lab is with sophisticated instruments such as laminar air flow chamber, and CO2 incubator, inverted microscope, Vis Spectrophotometer. Table top centrifuge and Waterbath. Further, Clean Room facility is also avalable in Biochemistry lab.

Microbiology Laboratory   

This lab will enable students to understand and describe staining characteristics, media selection, incubation conditions, colonial morphology, biochemical and serological reactions, and antibiotic sensitivity patterns of routine Bacteria, Viruses, and Parasites etc. students gain acquaintance with the microbial technology skills by working with state of art equipment like Laminar Flow Chamber, Orbital Shaking Incubator, Vertical Autoclave etc,. Special feature of this lab is well equiped with BSL II, Oribital shaker, 4oC Refrigerator, Water bath and Hot air oven.

Molecular Biology and Genetic Engineering Laboratory   

The objective of this lab is to provide a working knowledge of fundamental and advanced techniques like PCR, Blotting Techniques and Electrophoresis etc., that are used for the separation of amplification and cloning of isolated DNA into various types of vectors. The screening of such recombinants is carried out. Also the demonstration of mutagenesis helps the students to understand the mutation phenomenon. Special features of this lab is well equipped with advanced equipments viz., thermal cycler, Refrigerated microfuge, orbital shaking incubator, SDS PAGE, -20 oC Deep Freezer, Ice flaker, gel rocker, Vortex mixer, Microwave oven, 4 oC refrigerator, Western Blot apparatus.

Tissue Culture and Bioprocess Laboratory   

in this laboratory student will be taught to carry out in vitro creation of new plantlets and thereby learn the art and science of plant multiplication. With the aid of available modern trend and instruments, students will practice the various steps involved in the application of this technology like - stock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimatization and growing in sterile cultures to maintain parent lines. Students are exposed to clinical medicine and will be assigned laboratory exercises for acquainting them with the latest advances in immunological techniques today. Special features of this lab is well Equipped with laminar air flow, Refrigerator, Autoclave, Tissue Culture rack with illuminated tubelights with glass transparent shelves. LPG gas cylinder connected- burner to maintain sterile hood for explant to transplant.

Networking Facility funded by DST-FIST (Project Lab-I)   

The Networking Facility supported by DST-FSIT with Rs. 5.00 lakhs to procure 10 desktops with 4GB Graphic card for viewing the protein-ligand interactions in three dimensional posture. In addition, to screen the 3D view of biomolecules, a 55 inch LCD TV is also procured. The details of DST-FIST are as mentioned; Sanction Order No. : SR/FST/LSI-576/2013 (C).

Center of Excellence in Bio-resource utilization and management (Project Lab-II)   

The Center of Excellence in Bio-resource utilization and management is provided by VFSTR to provide state-of-art-facilities for students and scholars. The advanced instruments namely ELISA, PCR, HPLC, Sonicator and UV-Vis Spectrophotometer, Fluorescence Spectrophotometer and Gel Doc that are regularly being used by the students for the academic project works.

Brief description of Facilities in CoE (Project Lab-II)
Instrument: High Performance Liquid Chromatography

High performance Liquid Chromatography is a powerful tool popularly used in analysis of chemical compounds. It allows the user to spend minimal sample size and reap maximum results. The chemical compounds are analysed by high-efficient and sensitive photo diode array detector (DAD).

Instrument: UV-Vis Spectrophotometer

Cary 60 is a cuvette based versatile spectrophotometer capable of reading samples from 190 nm to 1100 nm with 1 nm increment. The system is also fitted with Peltier device for temperature regulation ranging from 0 OC to 100 OC to study temperature dependent chemical kinetics.

Instrument: Fluorescence spectrophotometer

Cary eclipse is a cuvette based highly sensitive and efficient fluorescent spectrophotometer. It can handle fluorescence, phosphorescence, chemi/bio-luminescence, and time resolved phosphorescence measurements very easily. The software is very user friendly and application specific.

Instrument: Inverted Fluorescence microscope

Inverted fluorescence microscope is a user-friendly and ergonomic tool for visualising animal cells under fluorescence and phase contrast fields. The microscope is fitted with 4 different filters viz., red, blue, green and violet that covers a wide range of fluorophores and gives the user a greater choice for dye selection. It has got high-quality objecitves-4X, 10X, 20X and 40X. The stage of the microscope is interchangeable to accommodate different kinds of culture wares.

Instrument: Upright Fluorescence Microscope

It is an ergonomic bright field and fluorescence microscope used for teaching, training and routine applications. It is fitted with 4X, 10X, 40X and 100X (oil immersion) objectives. Two fluorescence filters-blue and green provide fluorescence fields to work with different experiments. The microscope is also fitted with Olympus digital camera for efficient digital imaging and documentation.

Instrument: Microtiter Plate Reader

ELx800 is a filter based absorbance reader for 96 well plates. It measures optical density, absorbance and transmittance at 405nm, 490nm and 630nm. It is suitable for applications in experiments pertaining to clinical, biotechnology research and pharmaceutical laboratory.

Instrument: Microtiter Plate Reader

Multiskan FC is a monochromator based reader capable of reading 96 well and 384 well plates at wavelengths ranging from 340 nm to 850 nm. It is also equipped with shaking and incubation up to 50 OC for kinetics studies. Various assays such as Immunoassays (ELISA), protein assays, endotoxins, cytotoxicity and proliferation assays, enzyme assays and growth curves can be performed with ease using the SkanItTM Software.

Instrument: Sonicator

Sonicator is a probe equipment used for efficient disruption of materials. It is suitable for breaking Bacterial cell, bacterial spores, Animal & plant Cells. Also used for degassing, emulsifying, Cleaning, Mixing etc.

Instrument: UV-VIS Spectrophotometer

UV-1800 is a compact cuvette based UV-VIS spectrophotometer that can be used either as a stand-alone instrument or as a PC-controlled instrument. It offers high resolution of 1nm wavelength reading ranging from 180 nm to 1100 nm. Users cananalyse data on a PC using UVProbe software. In addition, data for spectra and time-course curves can be displayed and saved with commercial spreadsheet software.

Animal House Facility   

Using the animal house facility small laboratory vertebrates are developed as models for immunological, malignant, fibrosis and neurological disorders using the animal house facility. Animal house is registered with Committee for Purpose of Control and Supervision of Experiments on Animals (CPCSEA), MHRD bearing the registration number 2046/PO/ReBi/S/18/CPCSEA. Fully functional with separate housing rooms for mice, rats, guinea pigs, rabbits and hen. All cubicles are equipped with air conditioning facility, lighting, dehumidifier, compound microscope, refrigerator and incinerator. Snake pit track is desinged around the animal house. The additional facilities like store room, experimental room tap and water connection are made available.

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